Workshop on single-particle cryo-EM and tomography

Prato, Italy
9th - 14th April 2018

 Registration closed.

Course overview:

Cryogenic electron microscopy (cryo-EM) in conjunction with computerised image processing now enables the determination of high-resolution structures of macromolecular assemblies that have resisted X-ray crystallography and other approaches. The resolution revolution witnessed over the past decade has been fuelled by improved instrumentation, including extremely stable transmission electron microscopes and direct electron detectors with unprecedented sensitivity and resolution, as well as improved algorithms and software technologies that utilize modern heterogeneous computer architectures efficiently. Single-particle analysis can now be used routinely to produce maps of a quality that allows accurate modelling of the atomic structures of proteins. Tomography in conjunction with subtomographic averaging has broken the 1nm resolution barrier. In this workshop, we will provide in-depth training in image processing of electron micrographs in the areas of single-particle analysis and subtomographic averaging. The training will include a series of lectures covering the principles of image formation, the image processing methods typically applied and the latest technologies. The practical sessions will follow a tutorial for single-particle analysis and one for subtomogram averaging in order to provide the students with a complete overview of the possibilities currently available for structural determination with cryo-EM.

Selection criteria:

The course is intended for PhD students and researchers that are actively performing cryoEM or would like to start in order to bring their projects to the next level.
Applications should include: a short description of current work, future plans and an explanation of why attendance at the course would be beneficial to the applicant's research projects.
All applicants are encouraged to submit an abstract describing a project they are currently working on, and present a posted during the course.


The workshop will be heald at the Monash University Prato Centre in Prato, Tuscany, Italy.
Due to its rich history, Prato features a plethora of interesting structures such as the Emperor’s Castle and the Chiesa di San Francesco. Furthermore, due to its location, Prato is in close proximity to various natural areas of beauty such as the Parco dei Laghi and offers various opportunities for those who love the outdoors and adventure.


650 Euro Academics

1000 Euro Industry

Registration includes:

-  accommodation in double room (check in on the 9th to check out on the 14th)

-  lunch

-  coffee

-  drinks/refreshments during poster sessions.

Poster specifications:

Please bring one printed copy of your poster, size A0, portrait format.
The 4 best posters will be selected and those participants will have the opportunity to give a 15min oral presentation.

Confirmed speakers:

Pawel Penczek, UTHealth Medical School, Huston,TX, USA
Stefan Raunser, Max Planck Institute for Structural Biology, Dortmund, Germany
Misha Kudryashev, Max Planck Institute for Biophysics, Goethe University of Frankfurt.
Timothy Grant, Janelia Research Campus, Ashburn, VA, USA
Alex de Marco, Monash University, Melbourne, Australia
Alexis Rohou, Genentech, San Francisco Bay Area, CA, USA
Hans Elmlund, Monash University, Melbourne, Australia
Daniel Castano Diez, Biozentrum, University of Basel, Basel, Switzerland
Friedrich Foerster, Utrecht University, Nederlands
Miroslava Schaffer, The Max Planck Institute of Biochemistry, Munich, Germany


The organizing committee

Alex de Marco
Dominika Elmlund
Hans Elmlund

Day 1:


12.00pm – 3.00pm:


3.00pm – 4.00pm: 

"Introduction to cryo-electron microscopy"
  Alex de Marco

4.00pm – 5.00pm:

"Image formation and contrast transfer function"
  Alexis Rohou

5.00pm - 5.30pm:

 Coffee break

5.30pm - 6.30pm:

"Fundamentals of image reconstruction from projection images"
  Pawel Penczek

6.30pm - ?

 Welcome mixer

Day 2:


8.30am - 9.30am

"Resolution estimation and image restoration"
  Timothy Grant

9.30am – 10.30am:

"Analysis of single-particle projections in 2D and ab initio 3D reconstruction"
 Hans Elmlund

10.30am - 11.00am:

 Coffee break

11.00am - 12.00pm:

"Principles of subtomogram averaging"
  Misha Kudryashev

12.00pm - 1.00pm


1.00pm - 6.00pm


6.00pm – 7.00pm:

  Poster session I

Day 3:


8.30am - 9.30am

"3D classification in single-particle tomography"
  Daniel Castano Diez

9.30am – 10.30am:

"Template matching in tomography"
  Friedrich Foerster

10.30am –11.00am:

  Coffee break

11.00am - 12.00pm:

"Refinement and 3D classification in single-particle cryo-EM"
  Stefan Raunser

12.00pm - 1.00pm


1.00pm - 6.00pm


6.00pm – 7.00pm:

  Poster session II

Day 4:


8.30am - 9.30am

 "Sample preparation for cryo-EM"

10.30am – 6.00pm:


Day 5


10.30am – 6.00pm:

"Correlative light-electron microscopy"
  Miroslava Schaffer

12.00pm - 6.00pm


6.00pm - ?

  Workshop dinner

 Registration closed. <