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Workshop on cryo-EM and single-particle analysis


1-3 February 2017
ARC Centre of Excellence in Advanced Molecular Imaging
EMBL Australia
Monash University
Melbourne, Australia



Workshop schedule

 

 

Day 1:

 

9am – 12.30pm:

SIMPLE 3.0 part 1

12.30pm – 1.30pm:

lunch

1.30pm – 5.30pm:

SIMPLE 3.0 part 2

10.30am & 3.30pm:

coffee breaks

 

 

Day 2:

 

9am – 12.30pm:

EMAN 2.2 part 1

12.30pm – 1.30pm:

lunch

1.30pm – 5.30pm:

EMAN 2.2 part 2

10.30am & 3.30pm:

coffee breaks

 

 

Day 3:

 

9am – 12.30pm:

SCIPION part 1

12.30pm – 1.30pm:

lunch

1.30pm – 5.30pm:

SCIPION part 2

10.30am & 3.30pm:

coffee breaks

 

 

EM suite tours:

 

10 people/tour

Day 1&2 during breaks

 

 

BBQ party:

Day 2 @5.30pm - ?

 

 

General info & directions

 

Meeting point & registration:

Day 1 8.30am - 8.45am in building 75 on Monash Campus in Clayton next to Cinque Lire café. From there we will go to the computer labs.

 

Google maps:

 

Paid parking structure:

2min walk from the café

 

Campus map with both locations marked with large orange stars:

campus.pdf

 

LostOnCampus app:

https://studentvip.com.au/lost-on-campus

 

If you are late or got lost please call:

Sarah: +61 (0) 401 316 816

Dominika: +61 (0) 450 899 171

 

There is free wifi on campus.

Please take a sweater, computer labs are chilly.

Workshop material

 

Scope:

We will cover basics of cryo-EM theory and image processing. For those who like equations and want to understand image formation in more depth, please download: image_formation_theory.pdf

 

SIMPLE 3.0

SIMPLE 3.0 is currently in beta-testing and will be made available to the workshop participants. For general info about SIMPLE package see http://simplecryoem.com

workshop tutorial: simple3.0_tutorial.pdf
SIMPLE 3.0 manual: simple3.0manual.pdf

 

EMAN 2.2

EMAN2.2 is the newest version of EMAN, realesed 27/1/2017, for more info see: http://blake.bcm.edu/emanwiki/EMAN2/Tutorials#Video_tutorials_and_discussions

workshop tutorial: eman2.2_tutorial.pdf

 

SCIPION

SCIPION integrates many software packages for cryo-EM image processing under unified interface. For more info see: http://scipion.cnb.csic.es/m/home/

workshop tutorial: scipion_tutorial.pdf

 

Other programs that will be used:

CTFFIND4

UCSF Chimera

 

Linux tutorials (1-3):

http://www.ee.surrey.ac.uk/Teaching/Unix

 

evaluation.html

 

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